(Draxin) C1ORf187 from MyBioSource.com

Alternate nomenclature/Accession #: Dorsal repulsive axon guidance protein, Chromosome 1 Open reading frame 184 The secreted protein discovery initiative (SPDI) was established to identify novel secreted and trans-membrane proteins. The isolation of full-length cDNA clones for each of these candidate genes resulted in the identification of >1000 novel proteins. A total of 256 of these cDNAs are still novel, including variants and novel genes, per the most recent GenBank release version. The success of this large-scale effort was assessed by a bioinformatics analysis of the proteins through predictions of protein domains, subcellular localizations, and possible functional roles. The SPDI collection should facilitate efforts to better understand intercellular communication, may lead to new understandings of human diseases, and provides potential opportunities for the development of therapeutics. Chromosome 1 is gene-dense, with 3141 genes and 991 pseudogenes and many coding sequences overlap. The rearrangement and mutations in chromosome 1 are prevalent in cancer and many other diseases. Fine scale recombination occurs in hotspots of varying intensity along the sequences and these rearrangement and mutations are enriched near genes (1). One of the gene on chromosome 1 codes for a secreted protein Draxin, that acts as chemorepulsive axon guidance molecule required for the development of spinal cord and forebrain commisures (2). There are several axonal guidance proteins including netrins, semaphorins, ephrins, slits and morphogens such as hedgehox, Wnts and bone morphogenetic proteins (BMPs) that are involved in development of nervous system. However, immense complexity of CNS makes it likely that there are several more factors that are yet to be identified. Using signal sequence trap screening, a recently identified dorsal repulsive axonal guidance protein, draxin from motoneurons, floorplate and roof plate of chick embryo is identified (3). Transient expression of draxin was noted during CNS and spinal cord development. In mouse draxin is expressed in several brain regions including cortex, olfactory bulb, mid-brain and pontine nuclei of the post natal day 0 mouse brain. Deduced amino acid sequence analyses of draxin suggest no homology with any characterized axonal guidance or morphogens. The chick draxin consists of amino terminal signal peptide sequence but no membrane anchoring motifs which suggest draxin is a secreted protein. Ectopic expression of draxin inhibits growth and disrupts the routing of commissural axons an is required for development of midline crossing forbrain commisures. It is suggested that draxoim may be involved in the formation of indusium grieseum glia (2). MyBioSource has made rabbit anti-Draxin-antibodies using peptide taken unique region from near the carboxy-terminal end of the protein. The antigenic peptide was post-synthetically modified and covalently coupled to achieve desired antigenicity before using it as an immunogen to immunize rabbits. The rabbit antibodies obtained were isolated and purified on an immobilized antigen based affinity chromatographic matrix before stabilizing them in antibody stabilization buffer. Draxin antibodies labels Draxin protein as a single 41kDa band in PC-Draxin samples. Limited quantity of blocking peptide and Western blot positive controls for draxin in ready-to-use buffer (Cat PC-Drax) is also available, please inquire before placing order. MyBioSource will conjugate this antibody to fluorophores, secondary enzymes, or biotin, inquire for pricing and availability. MyBioSource has made a number of antibodies against axonal guidance factors and morphogens, for a complete